期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：2
页码：298-301
DOI：10.1073/pnas.71.2.298
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The ganglioside composition of a nonproducer subclone (derived from BALB/c 3T3 mouseembryo cells) transformed by Kirsten murine sarcoma virus was drastically altered compared to the nontransformed parental clone. The transformed clone was unable to synthesize the mono- and disialogangliosides, GM1 and GD1a, due to the complete absence of a specific galactosyltransferase. The lack of this enzyme activity was established by sensitive radiochemical and enzymological techniques in vivo and in vitro.
