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文章基本信息

  • 标题:In Vitro Synthesis of Ribosomal Proteins Directed by Escherichia coli DNA
  • 本地全文:下载
  • 作者:E. Kaltschmidt ; L. Kahan ; M. Nomura
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:2
  • 页码:446-450
  • DOI:10.1073/pnas.71.2.446
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:In vitro synthesis of a number of E. coli 30S ribosomal proteins has been demonstrated in a cell-free system consisting of ribosomes, initiation factors, RNA polymerase, a fraction containing soluble enzymes and factors, and E. coli DNA. DNA-dependent synthesis of the following 30S proteins has been demonstrated: S4, S5, S7, S8, S9, S10, S13, S14, S16, S19, and S20.
  • 关键词:ribosome reconstitution ; radio-immunodiffusion ; protein identification
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