标题：Transformation by Simian Virus 40 of Spleen Cells from a Hyperimmune Rabbit: Demonstration of Production of Specific Antibody to the Immunizing Antigen
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：2
页码：263-264
DOI：10.1073/pnas.71.2.263
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [125I]S3 polysaccharide-protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti-S3 antibodies of the donor rabbit. These observations strongly suggest that a normal committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.
