期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：3
页码：793-796
DOI：10.1073/pnas.71.3.793
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Prothoracic glands of tobacco hornworm larvae cultured in vitro secrete into the culture medium a substance which was active in ecdysone bioassays and determined to be ecdysone-like by radioimmunoassay. The prothoracic glands appear to be the sole source of this substance. The material was identified as [α]-ecdysone by thin-layer, gas-liquid, and high-resolution liquid chromatography and mass spectrometry. No other active moulting principles were present in the medium. It appears that the prothoracic gland secretes a prohormone, [α]-ecdysone, which is subsequently converted into the active moulting hormone, {beta}-ecdysone, in other insect tissues.
关键词：insect moulting hormone ; steroids ; organ culture ; radioimmunoassay
