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  • 标题:Association of DNA-dependent and -independent Ribonucleoside Triphosphatase Activities with dnaB Gene Product of Escherichia coli
  • 本地全文:下载
  • 作者:Sue Wickner ; Michel Wright ; Jerard Hurwitz
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:3
  • 页码:783-787
  • DOI:10.1073/pnas.71.3.783
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Preparations of E. coli dnaB gene product contain ribonucleoside triphosphatase activity that is stimulated 10-fold by DNA. The products of the triphosphatase activity are nucleoside diphosphates and Pi. The dnaB complementing activity in the {phi}X174 DNA-dependent system and these triphosphatase activities copurify over the last 20-fold of an extensive (about 40,000-fold) purification procedure. Acrylamide gel electrophoresis of the purified material shows a single band of protein coincident with eluted dnaB complementing and DNA-dependent and -independent nucleoside triphosphatase activities.
  • 关键词:DNA replication ; ϕX174 DNA ; ATP
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