期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：3
页码：947-951
DOI：10.1073/pnas.71.3.947
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The transducing phage {lambda}h80dlac carries the lac operator, whereas wild-type {lambda}h80 does not. We find that in high salt (0.18 M KCl), ultraviolet radiation causes the formation of a very stable complex between repressor and 5-bromodeoxyuridine (BrdU)-substituted {lambda}h80dlac but not to BrdU-{lambda}h80 DNA. Studies with inducers of the lac operon confirm the specificity of attachment. In low slat (0.01 M KCl), ultraviolet radiation will also attach repressor nonspecifically to BrdU-{lambda}h80 DNA. The stability of the complex suggests that covalent bonds are formed. We also report that another regulatory protein, the catabolite gene activator protein, can be attached similarly to DNA.
关键词：catabolite gene activator protein ; protein-DNA interaction ; nitrocellulose-filter assay
