首页    期刊浏览 2024年12月01日 星期日
登录注册

文章基本信息

  • 标题:A Mutant of Escherichia coli Defective in Leucyl, Phenylalanyl-tRNA-Protein Transferase
  • 本地全文:下载
  • 作者:R. L. Soffer ; M. Savage
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:3
  • 页码:1004-1007
  • DOI:10.1073/pnas.71.3.1004
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A mutant of E. coli that lacks leucyl,-phenylalanyl-tRNA-protein transferase (EC 2.3.2.6 ) has been isolated. Ability to produce the two activities could be introduced into the mutant from an F' strain whose episome contains genetic material located between 45 and 54 min on the E. coli chromosome. When grown into stationary phase and resuspended in minimal medium with glycerol, the mutant exhibited a marked lag before resuming growth. Revertants that did not show this lag were selected and were found to have regained both transfer activities. Extracts of wild-type, mutant, and revertant strains were compared as acceptors for the enzymatic transfer of radioactive phenylalanine. Analysis of the labeled polypeptides by disc gel electrophoresis indicated that certain potential acceptors may be preferentially acylated in vivo. These data provide genetic confirmation that the same enzyme protein catalyzes the transfer of leucine and phenylalanine and suggest that leucyl,phenylalanyl-tRNA-protein transferase is involved in a growth regulatory mechanism.
  • 关键词:regulation of growth ; post-translational modification
国家哲学社会科学文献中心版权所有