期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：3
页码：872-874
DOI：10.1073/pnas.71.3.872
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Human serum dopamine-{beta}-hydroxylase (3,4-dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase, EC 1.14.17.1 ) has been measured in a normal adult population by both enzymatic assay and by radioimmunoassay. The levels of enzymatic activity varied over a very wide range from 0.6 units/ml to 85 units/ml. In contrast, the amount of total dopamine-{beta}-hydroxylase protein present in these samples, as measured by radioimmunoassay, varied only over a 3-fold range: from 11.7 {micro}g/ml to 34.4 {micro}g/ml. From an analysis of the purified enzyme, the percentage of serum dopamine-{beta}-hydroxylase that was catalytically active was estimated to be small and variable.
