期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：6
页码：2544-2548
DOI：10.1073/pnas.71.6.2544
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Long-lived, immunologically vigorous (C3Hf x Af)F1 hybrids were produced after lethal irradiation by administration of spleen cells from C3Hf or syngeneic donors. Further, neonatally thymectomized C3Hf or Af strain donors reconstituted irradiated C3Hf or (C3Hf x Af)F1 hosts. In addition, C3Hf spleen cells from nonthymectomized 10- to 15-day-old donors protected irradiated hybrid mice, but Af cells of young mice as well as of older mice produced graft-versus-host reaction and early death in irradiated C3Hf or (C3Hf x Af)F1 hybrids. Abrogation of secondary disease by treatment of irradiated mice with spleen cells from allogeneic neonatally thymectomized mice is possibly attributable to diminished immunologic competence of the cells grafted, followed by the development of immunological tolerance of the donor cells. Donor cells, receiving thymus influence in the recipient host after transplantation, could explain the long-lived immunologically vigorous radiation chimeras that did not experience graft-versus-host reactions. The findings of this study help to understand the differential susceptibility of Af and C3Hf mice to development of tolerance to one another's antigens observed in prior investigations. It appears that, in these mice, the host thymus influences the maturation of the spleen cells from young mice or from neonatally thymectomized mice. However, this influence was often greater in mice given 767 rads than in those given 1046 rads. This differential influence is possibly attributable to irradiation damage to the thymus produced by the higher dose of irradiation. Spleen cells from neonatally thymectomized mice can be differentiated and expanded by the thymus of the host. The differential susceptibility of T1, early differentiation stages, of thymus-dependent lymphocytes and T2, late differentiation stages, of thymus-dependent lymphocytes to tolerance induction and immunostimulation, respectively, are proposed as the bases for these otherwise paradoxical influences.
