期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：6
页码：2268-2272
DOI：10.1073/pnas.71.6.2268
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Actin purified from mouse fibroblasts by sodium dodecyl sulfate gel electrophoresis was used as antigen to obtain an antibody in rabbits. The elicited antibody was shown to be specific for actin as judged by immunodiffusion and complement fixation against partially purified mouse fibroblast actin and highly purified chicken muscle actin. The antibody was used in indirect immunofluorescence to demonstrate by fluorescence light microscopy the distribution and pattern of actin-containing filaments in a variety of cell types. Actin filaments were shown to span the cell length or to concentrate in "focal points" in patterns characteristic for each individual cell.
关键词：immunofluorescence ; microfilaments ; sodium dodecyl sulfate gel electrophoresis
