期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：6
页码：2367-2371
DOI：10.1073/pnas.71.6.2367
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Satellite phage P4 directs the capsid proteins of its helper phage, P2, to form a head which is only one-third the size of the normal P2 head. The P2 head contains a genome of molecular weight 22 x 106, while the small P4 head contains a genome with a molecular weight of only 7 x 106. We have used in vitro DNA packaging to test whether P2 and P4 phage head sizes are determined by DNA size. The small DNA of satellite phage P4 added to a P2-infected cell extract was packaged primarily into particles containing three copies of the P4 genome. This process occurred with approximately the same efficiency as P2 DNA packaging in the same cell extract. In contrast, the large DNA of P2 was packaged 300-fold less efficiently than the small DNA of P4 in an extract derived from P4-infected, P2-lysogenic cells. These results suggest that DNA size is not sufficient to determine head size. The results are compatible with DNA packaging via the filling of preformed empty capsids.
关键词：bacteriophage P4 ; bacteriophage P2 ; morphogenesis ; DNA condensation
