期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1974
卷号:71
期号:6
页码:2372-2376
DOI:10.1073/pnas.71.6.2372
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:A precursor head of phage lambda is synthesized after induction of cells lysogenic for {lambda}D-F- and {lambda}A- (head-defective) mutants. This precursor head can be assayed by complementation in vitro and can be purified by CsCl gradient centrifugation and sucrose gradient sedimentation. The precursor head contains no DNA and has the same dimensions as the petit {lambda} particle. It can be packed with {lambda} DNA in an extract from induced Escherichia coli lysogenic for a {lambda}E- mutant.
关键词:morphogenesis ; DNA condensation ; in vitro complementation