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  • 标题:Activity of Empty, Headlike Particles for Packaging of DNA of Bacteriophage λ In Vitro
  • 本地全文:下载
  • 作者:B. Hohn ; T. Hohn
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:6
  • 页码:2372-2376
  • DOI:10.1073/pnas.71.6.2372
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A precursor head of phage lambda is synthesized after induction of cells lysogenic for {lambda}D-F- and {lambda}A- (head-defective) mutants. This precursor head can be assayed by complementation in vitro and can be purified by CsCl gradient centrifugation and sucrose gradient sedimentation. The precursor head contains no DNA and has the same dimensions as the petit {lambda} particle. It can be packed with {lambda} DNA in an extract from induced Escherichia coli lysogenic for a {lambda}E- mutant.
  • 关键词:morphogenesis ; DNA condensation ; in vitro complementation
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