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  • 标题:In Vitro Repair of Apurinic Sites in DNA
  • 本地全文:下载
  • 作者:W. G. Verly ; F. Gossard ; P. Crine
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:6
  • 页码:2273-2275
  • DOI:10.1073/pnas.71.6.2273
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Apurinic sites disappear from DNA during an incubation of the DNA with the Escherichia coli endonuclease specific for apurinic sites, DNA polymerase I (EC 2.7.7.7 .), and T4 ligase (EC 6.5.1.1 ). Omission of any one of these three enzymes and, in particular, omission of the endonuclease specific for apurinic sites prevents this in vitro repair.
  • 关键词:DNA depurination ; endonuclease specific for apurinic sites ; DNA repair
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