期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：6
页码：2295-2299
DOI：10.1073/pnas.71.6.2295
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The purification of a growth factor from bovine pituitary glands that controls the division of an ovarian cell line maintained in tissue culture is reported. Extraction of bovine pituitary glands at pH 4.5 followed by ammonium sulfate precipitation, chromatography on carboxymethylcellulose, and gel filtration on Sephadex G-50 gives yields of ovarian growth factor of about 7 mg/kg of glands. Contamination by other pituitary hormones was not detected. The preparation obtained has been characterized with respect to biological activity, molecular weight, and amino-acid composition. The implication of this growth factor in the development of ovarian tumors is discussed.
关键词：pituitary hormones ; ovarian tumor ; growth control
