期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：7
页码：2761-2765
DOI：10.1073/pnas.71.7.2761
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：RNA polymerase was precipitated from extracts of Bacillus subtilis infected with phage SP01 by antiserum prepared against core RNA polymerase. As shown by sodium dodecyl sulfate gel electrophoresis, the precipitates contained at least five new polypeptides not present in uninfected bacteria, in addition to the known subunits of RNA polymerase. The molecular weights of these polypeptides are (1) 85,000; (II) 40,000; (III) 28,000; (IV) 25,000; and (V) 23,000. Four of the polypeptides (I, III, IV, and V) co-purified with RNA polymerase through gel filtration and phosphocellulose chromatography. A pulse-chase experiment indicated that all five polypeptides are synthesized de novo after infection. The synthesis of polypeptides II, III, and IV commences almost immediately after infection, while polypeptides I and V first appear several minutes later. A sus mutant blocked early in transcription, susF21 [Fujita, et al. (1971) J. Mol. Biol. 57, 301-317] failed to induce polypeptides I, IV, and V, while two other mutants, susF4 and susF14, blocked late in transcription both failed only to induce polypeptide V.
