期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：7
页码：2679-2683
DOI：10.1073/pnas.71.7.2679
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：For study of the basis of an X-linked form of gout in man, several clonal lines deficient in hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8 ) were selected from the human lymphoblast line WI-L2 by spontaneous and mutagen-induced resistance to 10 {micro}M 8-azaguanine. Three groups could be defined: (1) clones with less than 1% of normal enzyme activity, unable to incorporate [3H]hypoxanthine detectable by radioautography, unable to tuilize exogenous hypoxanthine as a source of purines, and showing a 2- to 4-fold accelerated rate of production of early intermediates in de novo purine biosynthesis; (2) clones with 56-63% of normal enzyme activity, decreased incorporation per cell of [3H]hypoxanthine measured by radioautography, able to utilize exogenous hypoxanthine, and showing 1.2- to 2.8-fold purine overproduction; (3) clones with 10-15% of normal enzyme activity, able to utilize hypoxanthine but not incorporating amounts detectable by radioautography, and showing a 2.3- to 2.5-fold increase in purine biosynthesis. Resistant clones generated by ICR 191 mutagenesis resembled Group 1 clones. Heat inactivation studies in crude extracts from certain clones in Group 2 suggest a structural gene mutation, but no qualitative alteration in enzyme could be detected by starch gel electrophoresis. These phenotypes have persisted over at least 300 generations of nonselective growth, with retention of a diploid karyotype.
