期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：7
页码：2614-2617
DOI：10.1073/pnas.71.7.2614
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have isolated a specialized transducing phage carrying the polC locus of E. coli K-12. A strain of E. coli lacking the lambda attachment site was infected with bacteriophage {lambda}. Lysogens carrying {lambda} at the tonA locus were isolated by selecting {lambda}-immune, T5-resistant strains. Transducing phages of dapC, dapD and polC, which map within 0.2 min of tonA, were obtained in lysates prepared from two of the lysogens. The isolated phage, {lambda}dpolC, is defective but can transduce four different polC temperature-sensitive mutants. After lysogenation with the transducing phage, DNA polymerase III activity is restored to normal levels in extracts of a polC strain lacking polymerase III activity. However, attempts to obtain increased levels of DNA polymerase III in extracts of induced lysogens carrying {lambda}dpolC have been unsuccessful.
关键词：λ attachment site ; tonA ; λ dpolC ; DNA polymerase III
