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  • 标题:Fusion and Compatibility of Camphor and Octane Plasmids in Pseudomonas
  • 本地全文:下载
  • 作者:George I. N. Chou ; Dvorah Katz ; I. C. Gunsalus
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:7
  • 页码:2675-2678
  • DOI:10.1073/pnas.71.7.2675
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The octane (OCT) plasmid in Pseudomonas putida derived from the {omega}-hydroxylase-carrying strain of Coon and coworkers is transferable to the camphor (CAM) plasmid-bearing strain by conjugation or by transduction. While the majority of the Cam +Oct+ exconjugants segregate Cam+ or Oct+ cells, exconjugants with stable Cam +Oct+ phenotype (CAM-OCT) can be detected at a low frequency. The transductants are all of the CAM-OCT phenotype. In the stable Cam +Oct+ strains, the OCT plasmid resembles the CAM plasmid with respect to curing by mitomycin C, transfer in conjugation, and reaction to ts (temperature-sensitive) mutation specifically affecting CAM plasmid replication. Therefore, it is suggested that certain regions of homology exist between the CAM and OCT plasmids that enable them to recombine to form a single plasmid, and to overcome the incompatibility barrier that prevents their coexisting.
  • 关键词:plasmid transduction ; recombination
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