期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：8
页码：2951-2955
DOI：10.1073/pnas.71.8.2951
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Hybridization to the separated light (L) and heavy (H) strands of adenovirus 2 DNA in 50% formamide at 37{degrees} was used to isolate undegraded virus-specific RNA molecules from the polyribosomes of cycloheximide-treated human KB cells early after infection with adenovirus 2. About 20% of polyribosomal RNA labeled with [3H]uridine from 4 to 7 hr after infection was virus-specific. Twice as much labeled RNA was homologous to the L strand as to the H strand. Polyacrylamide gel electrophoresis of RNA selected with unfractionated adenovirus DNA resolved a major component of virus-specific RNA in the 19-20 S region of the gel and smaller amounts of viral RNA in two heterogeneous fractions migrating at 15-18 S and 21-26 S. Selection with individual DNA strands showed that the 19-20 S main size class of early mRNA consists of two homogeneous RNA species with slightly different mobilities, the transcripts from the L and H strand having molecular weights of 7.4 x 105 and 7.7 x 105, respectively. The 15-18 S RNA hybridized with the L strand and the 21-26 S RNA with the H strand.
关键词：DNA·RNA hybridization ; gel electrophoresis ; cycloheximide
