期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3385-3389
DOI：10.1073/pnas.71.9.3385
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Several polypeptides contained in the coat and the core of adenovirus type 2 particles have been identified as virus gene products. They are synthesized in vitro by a cell-free protein-making system programmed by adenovirus type 2 messenger RNA that has been hybridized with and eluted from virus DNA. Fractionation by size yields subpopulations of viral messenger RNA that differ in their coding specificities. The procedures outlined in this study may be used to establish a genetic map of adenovirus type 2.
关键词：polysomal RNA ; oligo(dT)-cellulose ; RNA·DNA hybridization ; Krebs II ascites system
