期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3560-3564
DOI：10.1073/pnas.71.9.3560
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The nucleotide sequence complexity of 60-70S RNA of Rous sarcoma virus was determined from the molar yields of 11 pure oligonucleotides of known chain length, obtained from [32P]RNA of Rous sarcoma virus by T1 RNase digestion and two-dimensional polyacrylamide gel electrophoresis. We calculate an apparent chain length of about 9800 nucleotides, corresponding to a molecular weight of 3.4 x 106. Assuming the molecular weight of 60-70S RNA of Rous sarcoma virus to be about 107, our data suggest that the virus genome consists of identical, or very similar, nucleotide sequences repeated three to four times.
关键词：Rous sarcoma virus ; T1-oligonucleotides ; two-dimensional gel electrophoresis
