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  • 标题:Simian Virus 40 T Antigen Binds to DNA
  • 本地全文:下载
  • 作者:R. B. Carroll ; L. Hager ; R. Dulbecco
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:9
  • 页码:3754-3757
  • DOI:10.1073/pnas.71.9.3754
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The T antigen of simian virus 40, which may play a role in the control of viral DNA replication, is recovered from nuclei of cells transformed by simian virus 40 in several forms sedimenting at different rates. The large molecular weight forms are converted to the smallest (5 S) form by high salt, suggesting that they differ in the degree of aggregation. All the forms of the antigen bind efficiently to double-stranded DNA-cellulose columns at pH 6.2 and low salt, and elute in two fractions: one at pH 8.0 and low salt, the other at pH 8.0 and high salt. The antigen has little affinity for single-stranded DNA.
  • 关键词:sedimentation ; multiple species ; dissociation ; denatured DNA ; native DNA
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