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  • 标题:Dissociation and Interaction of Individual Components of a Degradative Plasmid Aggregate in Pseudomonas
  • 本地全文:下载
  • 作者:A. M. Chakrabarty ; Denise A. Friello
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:9
  • 页码:3410-3414
  • DOI:10.1073/pnas.71.9.3410
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The transfer of the OCT plasmid from Pseudomonas oleovorans to Pseudomonas putida strain PpGl results in the acquisition of three independent replicons: OCT, factor K, and the MER plasmid. OCT is a nontransmissible plasmid harboring genes that code for the enzymes responsible for the degradation of n-octane. Factor K is a transfer plasmid capable of mobilizing OCT as well as chromosomal genes but incapable of enhancing transfer frequencies of other transmissible plasmids such as CAM, SAL, or RP-1. MER is a self-transmissible plasmid which can confer resistance to high concentrations of mercury salts. While OCT and MER are incompatible with CAM, factor K is compatible with it. Transmissible plasmids such as SAL, CAM, MER, or RP-1 cannot mobilize OCT to any significant extent, and exert strong repression on factor K-mediated transfer of chromosomal genes.
  • 关键词:transfer plasmid ; plasmid compatibility ; chromosome mobilization ; mercury resistance
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