期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1974
卷号:71
期号:9
页码:3527-3531
DOI:10.1073/pnas.71.9.3527
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:Transfer RNA5Lys was isolated and purified from Drosophila melanogaster. It was iodinated in vitro with sodium [125I]iodide and hybridized in situ to the polytene salivary gland chromosomes from Drosophila. Autoradiographs exposed three to four weeks allowed the localization of this tRNA to the right arm of chromosome 2 between bands 48F and 49A.
关键词:5S RNA ; 125I-iodination ; in situ hybridization