期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：11
页码：4278-4282
DOI：10.1073/pnas.71.11.4278
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A protein-synthesizing system has been established in vitro, in which tubulin is synthesized on polysomes isolated from brain and leg muscle of embryonic chick. The tubulin synthesized in vitro is characterized by (i) its electrophoretic mobility on sodium dodecyl sulfate-acrylamide gels (brain: 55,000 daltons; leg muscle: 53,000 daltons), and (ii) its ability to function as a microtubular subunit, as judged by its specific ability to participate in at least two polymerization and depolymerization steps in the microtubule assembly system in vitro.
