期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：11
页码：4579-4583
DOI：10.1073/pnas.71.11.4579
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A bacteriophage {lambda} strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the {lambda} DNA. These hybrid DNAs can infect nonrestricting Escherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this {lambda} strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages have been produced with E. coli DNA and Drosophila melanogaster DNA.
关键词：Eco RI restriction endonuclease ; DNA joining ; calcium transfection ; electron microscopy
