期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：12
页码：5088-5090
DOI：10.1073/pnas.71.12.5088
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A23187 is a carboxylic antibiotic that selectively transfers calcium, magnesium, and other divalent cations across biologic membranes. This ionophore was found to produce morphologic blast transformation, DNA synthesis, and mitosis in human lymphocytes. Several hours of ionophore-cell contact were necessary to produce optimal mitogenesis. The effects were highly dependent on the presence of extracellular calcium and much less dependent on extracellular magnesium. Lanthanum chloride prevented the development of the observed ionophore effects. Results are consistent with the hypothesis that under physiologic conditions the interaction of antigens or mitogens with specific receptors at the lymphocyte membrane initiates events that alter calcium fluxes and result in increased cytoplasmic calcium. Increased cytoplasmic calcium is postulated to play a central role in the generation of surface-to-nuclear signals that initiate the process of DNA synthesis and cell division.
关键词：calcium fluxes ; mitogenic signals ; human lymphocytes
