期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：12
页码：4783-4786
DOI：10.1073/pnas.71.12.4783
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Ribonucleic acid containing poly(adenylic acid) [poly(A)-RNA] is present in barley aleurone layers. This poly(A)-RNA becomes labeled with radioactive precursors of RNA during the incubation of isolated aleurone layers with or without gibberellic acid. However, the rate of synthesis of poly(A)-RNA is enhanced by gibberellic acid. This enhancement begins within 3-4 hr of addition of the hormone and reaches a maximum, which is about 50-60% over the control, 10-12 hr after addition of the hormone. Cordycepin inhibits total RNA as well as poly(A)-RNA synthesis in barley aleurone layers. However, cordycepin inhibits the hormone-controlled synthesis of [α]-amylase (EC 3.2.1.1 ) only if it is added 12 hr or less after gibberellic acid. The insensitivity of [α]-amylase production to cordycepin after 12 hr of gibberellic acid treatment suggests that [α]-amylase is translated from stable messenger RNA.
