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  • 标题:Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro
  • 本地全文:下载
  • 作者:S Wickner ; J Hurwitz
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1975
  • 卷号:72
  • 期号:3
  • 页码:921-925
  • DOI:10.1073/pnas.72.3.921
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro. This interaction was demonstrated as follows: (a) A complex of dnaB and dnaC(D) gene products was isolated by gel filtration; ATP specifically was required for isolation of the complex. (b) The DNA-independent ribonucleoside triphosphatase activity associated with dnaB gene product was inhibited by dnaC(D) gene product. (c) The dnaC(D) gene product was protected from inactivation by N-ethyl-maleimide by the combination of dnaB gene product and ATP; this protection required ATP specifically.
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