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  • 标题:Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments
  • 本地全文:下载
  • 作者:J B Lewis ; J F Atkins ; C W Anderson
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1975
  • 卷号:72
  • 期号:4
  • 页码:1344-1348
  • DOI:10.1073/pnas.72.4.1344
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Cytoplasmic RNA, isolated from cells late after infection by adenovirus type 2 and fractionated by hybridization to specific fragments of adenovirus DNA produced by cleavage with the endonuclease R-EcoRI, was used as template for protein synthesis in cell-free mammalian extracts. Each of the R-EcoRI fragments of DNA selects RNA that encodes specific subsets of the viral polypeptides. From the known order of the R-EcoRI fragments, the following partial map is deduced: (III, IIIa, IVa2, V, P-VII, IX), (II, P-VI), 100K, IV-where the relative order of the components enclosed in parentheses has not yet been determined.
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