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  • 标题:Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum
  • 本地全文:下载
  • 作者:M Clarke ; G Schatten ; D Mazia
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1975
  • 卷号:72
  • 期号:5
  • 页码:1758-1762
  • DOI:10.1073/pnas.72.5.1758
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin.
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