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  • 标题:Methylated, blocked 5 termini in HeLa cell mRNA
  • 本地全文:下载
  • 作者:Y Furuichi ; M Morgan ; A J Shatkin
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1975
  • 卷号:72
  • 期号:5
  • 页码:1904-1908
  • DOI:10.1073/pnas.72.5.1904
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Poly(A)-containing HeLa cell mRNA prepared from cells labeled with [methyl-(3)H]methionine or [(32)P]phosphate was found to contain a variety of methylated, blocked 5'-terminal structures of two general types: m(7)GpppN(7)-Np and m(7)GpppN(m)-N(m)-Np. In addition, about one-third of the [(3)H]methyl label was present in the N(6)-methyladenosine; this labeled nucleoside was not found in the 3'-terminal one-third of the mRNA chain and thus may also be in the 5' portion of the mRNA.
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