期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1975
卷号：72
期号：6
页码：2188-2192
DOI：10.1073/pnas.72.6.2188
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A cell-free system that promotes the excision of prophage lambda DNA has been established. The substrate for the reaction is phage DNA carrying two attachment sites, which, in vivo, undergoes intramolecular recombination between these sites. The in vitro recombination system is efficient; 25-35% of the substrate DNA undergoes recombination in 30 min. There is an absolute requirement for ATP; Mg++ and spermidine are stimulatory. RNA does not appear to be involved, nor can a role for DNA synthesis be demonstrated.
