期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1975
卷号：72
期号：8
页码：2984-2988
DOI：10.1073/pnas.72.8.2984
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Four chromatographically separable DNA-dependent RNA polymerases (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6 ) were partially purified from imaginal discs of Drosophila melanogaster. Their properties are similar to those described for RNA polymerases I and II isolated from other eukaryotes. In vitro incubation of discs with beta-ecdysone, juvenile hormone, or cycloheximide resulted in increased activity of RNA polymerase I. The increase was irreversible with beta-ecdysone incubation and removal but reversible with juvenile hormone incubation and removal. With beta-ecdysone, the rate of the increase in polymerase I activity paralleled the kinetics of ecdysone binding to discs and increases in the rate of precursor incorporation into RNA. A model to explain the increased acticity of RNA polymerase I is presented.
