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  • 标题:Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells
  • 本地全文:下载
  • 作者:J P Merlie ; A Sobel ; J P Changeux
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1975
  • 卷号:72
  • 期号:10
  • 页码:4028-4032
  • DOI:10.1073/pnas.72.10.4028
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Acetylcholine receptor, a component of the specialized muscle membrane, appears during the differentiation of embryonic myogenic cells in tissue culture. Demonstration of incorporation of the radioactive precursor L-[35S]methionine into purified receptor polypeptides is presented as evidence for its synthesis de novo. The identity of the purified radioactive species is established by cosedimentation of the [35S]receptor with [3H]alpha-toxin binding activity on sucrose gradients and by crossreaction with antiserum to purified acetylcholine receptor of Electrophorus electricus.
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