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  • 标题:Novel F prime factors able to replicate in Escherichia coli Hfr strains
  • 本地全文:下载
  • 作者:S Hiraga
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1976
  • 卷号:73
  • 期号:1
  • 页码:198-202
  • DOI:10.1073/pnas.73.1.198
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A novel type of F' plasmid that can replicate extrachromosomally in cells of Hfr strains has been isolated. Genetic analysis of these plasmids indicates that all carry the dnaA-bglA region of the E. coli chromosome as well as ilv+ used as a selective marker. It is suggested that a specific site, designated poh+ (permissive on Hfr), is located in this region, and is essential for these plasmids to replicate in Hfr cells. The most interesting explanation, among others, would be that the poh+ site represents the replication origin of the bacterial chromosome.
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