期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：2
页码：401-404
DOI：10.1073/pnas.73.2.401
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Staphylococcal nuclease (nucleate 3'-oligonucleotidohydrolase; EC 3.1.4.7 ) cleaved DNA within disrupted adenovirus particles into a regular series of fragments with a repeat unit of 200 base pairs. Since this pattern did not eppear when DNA alone was digested, we postulate that the orderly arrangement of core polypeptides protects discrete regions of DNA from nuclease attack. The 23 X 10(6) dalton adenovirus DNA molecule can accommodate 180 units of roughly 200 base pairs. Based on the stoichiometry of core polypeptides, we calculate that each repeat unit contains six copies of polypeptide VII and a single copy of polypeptide V. This model is bases on proposals for the structure of eukaryotic chromatin. Very brief nuclease digestion generated 1800 base pair fragments (1/20 of the adenovirus chromosome). This result is discussed in terms of a higher order folding of viral DNA within the virus particle.
