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  • 标题:The partial amino-acid sequence of an H-2K molecule
  • 本地全文:下载
  • 作者:E S Vitetta ; J D Capra ; D G Klapper
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1976
  • 卷号:73
  • 期号:3
  • 页码:905-909
  • DOI:10.1073/pnas.73.3.905
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Eighteen of the NH2-terminal 27 amino acids of a murine H-2K molecule have been assigned. The approach used was to label murine splenocytes with a single radioactive amino acid, isolate the H-2K molecule by specific immunoprecipitation, electrophorese the dissolved precipitate on sodium dodecyl sulfate polyacrylamide gels, and subject the isolated H-2K peak to amino-acid analysis and automated sequencing.
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