期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1976
卷号:73
期号:7
页码:2505-2509
DOI:10.1073/pnas.73.7.2505
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:HeLa cells infected with the nondefective adenovirus 2-SV40 hybrid virus (Ad2+ND2) have previously been shown to synthesize two SV40-specific proteins with molecular weights of 56,000 (56 K protein) and 42,000 (42 K protein). The present study demonstrates that these proteins are immunoprecipitable with serum from hamsters bearing SV40 tumors. By peptide analysis, it is shown that the 56 K and 42 K proteins share common amino acid sequences. Both proteins are metabolically stable in nuclei and plasma membranes but unstable in the cytoplasm, as shown by pulse-chase experiments and cell fractionation studies.