期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：10
页码：3408-3412
DOI：10.1073/pnas.73.10.3408
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The reverse transcriptase (RNA-directed DNA nucleotidyltransferase) from avian myeloblastosis virus is able to make an extensive, possibly complete, complementary DNA copy of intact poliovirus RNA. In the presence of high concentrations of deoxyribonucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, this DNA is the only species produced. Without these additives, however, a second size class of DNA is also synthesized. This material has a sedimentation coefficient between roughly 4 and 10 S and is produced later in the reaction, largely after synthesis of the larger complementary DNA has ceased. The smaller DNA consists primarily of material anticomplementary to the RNA template and contains a faithful and uniform representation of the viral sequences. It most likely arises by transcription of the larger DNA species.
