标题：Isolation, primary structure, and synthesis of α-endorphin and γ-endorphin, two peptides of hypothalamic-hypophysial origin with morphinomimetic activity
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：11
页码：3942-3946
DOI：10.1073/pnas.73.11.3942
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The isolation and primary structure of two peptides with morphinomimetic activity, obtained from an extract of porcine hypothalamus-neurohypophysis, are described. The amino acid sequence of the two peptides, named -endorphin and {gamma}-endorphin, was determined by mass spectrometry and densyl-Edman methods to be H-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-OH and H-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-OH, respectively. These correspond to the amino acid sequences present between residues 61 and 76 and residues 61 and 77 of the various {beta}-lipotropins. A third peptide also obtained in pure from in these studies was found to be an unstable salt of -endorphin.
关键词：opiates ; endogenous ligand ; mass spectrometry ; high pressure chromatography
