期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：2
页码：492-495
DOI：10.1073/pnas.74.2.492
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A multi-enzyme complex of fatty acid oxidation has been isolated from E. coli B cells and has been purified to near homogeneity by a simple two-step procedure. The complex exhibits thiolase (EC 2.3.1.9 ), enoyl-CoA hydratase (EC 4.2.1.17 ), and 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35 ) activities towards short-, medium-, and long-chain substrates. The complex has been estimated to have a molecular weight of approximately 300,000 and is apparently composed of two types of subunits with molecular weights of 78,000 and 42,000.
