期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：5
页码：2050-2053
DOI：10.1073/pnas.74.5.2050
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：When Drosophila melanogaster embryos were ligated at very early stages of nuclear multiplication (45-60 min after egg deposition) for the entire period of embryogenesis, egg fragments of variable sizes were able to continue development up to the hatching stage. These ligated embryos differentiated larval structures; anterior fragments formed larval head and posterior fragments formed larval abdominal structures. Ligation always prevented the differentiation of some of the intermediate larval bands whereas the terminal ones were always formed. Identical results were obtained when embryos (45-60 min after egg deposition) were temporarily ligated for only 5 min. However, puncturing with a fine glass needle immediately after temporary ligation through the ligated area could give rise to complete differentiation of all the bands. Since puncturing disrupted the barrier produced by ligation, we propose that interaction between anterior and posterior egg regions is necessary to allow complete development.
