期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：6
页码：2251-2255
DOI：10.1073/pnas.74.6.2251
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：When added to a sparse culture of 3T3 cells, a surface membrane-enriched fraction from 3T3 cells inhibited the rate of DNA synthesis in a time- and concentration-dependent manner. The membrane preparation had no effect on the rate of DNA synthesis of simian virus 40-transformed 3T3 cells. A similar membrane preparation from transformed cells had a lesser inhibitory effect on 3T3 cells and no effect on transformed cells. The inhibition by membranes was reversible. The data suggest that, when added to growing 3T3 cells, 3T3 surface membranes can mimic the effect of increasing cell density on DNA synthesis.
