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  • 标题:Potato spindle tuber and citrus exocortis viroids undergo no major sequence changes during replication in two different hosts
  • 本地全文:下载
  • 作者:Elizabeth Dickson ; T. O. Diener ; Hugh D. Robertson
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1978
  • 卷号:75
  • 期号:2
  • 页码:951-954
  • DOI:10.1073/pnas.75.2.951
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Potato spindle tuber viroid and citrus exocortis viroid, each purified from tomato (Lycopersicon esculentum) and from Gynura aurantiaca, were iodinated in vitro with 125I, digested with ribonuclease T1, and subjected to two-dimensional RNA fingerprinting analysis. With the exception of minor variations, each viroid retained its distinctive fingerprint pattern irrespective of the host species from which it was isolated. We conclude that the nucleotide sequences of these viroids are principally determined by the infecting viroid and not by the host.
  • 关键词:RNA fingerprinting ; iodine-125 ; viroid diseases
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