期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：4
页码：1652-1656
DOI：10.1073/pnas.75.4.1652
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The first step in the replication cycle of the single-stranded DNA phages is the conversion of the infecting positive strand circle to a duplex ring. This event involves the de novo initiation of a negative strand, using the infecting positive strand cycle as a template. The synthesis of the negative strand is in many respects analogous to the formation of a fragment during cellular DNA replication. In this paper we describe the initiation of the negative strand of bacteriophage G4. The data establish that, in vivo, the synthesis of the G4 negative strand is initiated at a specific site, which we have mapped on the 5400-base viral chromosome.
