期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：5
页码：2401-2405
DOI：10.1073/pnas.75.5.2401
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The effects of various anti-Ig antibodies on different B lymphocyte functions were investigated. With the proper accessory cofactor(s) derived from serum, anti-IgM antibodies induced a vigorous proliferative response in normal adult murine B cells, while polyspecific anti-Ig and anti-IgD had no effect. Without the required cofactor, all three anti-Ig antibodies were inhibitory for mitogenic responses. All three anti-Ig antibodies were also inhibitory for mitogen-induced antibody responses with or without the cofactor. Even with the required cofactor, neonatal B cells as well as adult C3H/HeJ B cells were not triggered into proliferation by anti-IgM. Finally, the cofactor required for anti-IgM-triggered mitogenesis was shown to be generated from serum by 2-mercaptoethanol and to be approximately 65,000 in molecular weight. These results indicate that, for at least some responses, B lymphocyte surface IgM molecules are involved in both triggering and suppression, depending both on the developmental state of the B cell and the presence or absence of accessory influences. In these experiments, IgD gave evidence only of being suppressive.
