期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：5
页码：2502-2506
DOI：10.1073/pnas.75.5.2502
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The unlabeled peroxidase-antiperoxidase method has been used with an antiserum against rat dopamine-beta-hydroxylase (DBH) to obtain a three-dimensional image of noradrenergic cell bodies and their processes in thick Vibratome sections of rat brain. This method stains DBH-positive neurons exclusively with a result similar to that of the Golgi method, which makes it possible to analyze the geometric plan of these neurons and their projections in the central nervous system. In 100-micron sections, DBH-positive axons can be followed over long distances, and the results indicate that their distribution in cerebral and cerebellar cortex is not diffuse but has a strict geometric order.
