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  • 标题:Proposed temperature-dependent conformational transition in D-amino acid oxidase: a differential scanning microcalorimetric study
  • 本地全文:下载
  • 作者:J M Sturtevant ; P L Mateo
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1978
  • 卷号:75
  • 期号:6
  • 页码:2584-2587
  • DOI:10.1073/pnas.75.6.2584
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A number of authors have reported observations on D-amino acid oxidase [D-amino acid: O2 oxidoreductase (deaminating), EC 1.4.3.3 .] that they have interpreted in terms of a temperature-dependent conformational transition having a van't Hoff enthalpy amounting to more than 1 cal per g of protein (1 cal = 4.184J). No indication of this transition is obtained by using a differential scanning calorimeter having a sensitivity considerably in excess of that required to detect such a transition. The implications of this discrepancy are discussed.
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