期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：6
页码：2689-2693
DOI：10.1073/pnas.75.6.2689
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Antibodies that recognized the prostaglandin (PG) E structure were elicited from rabbits. 9-Deoxy-9-methylene-PGF2alpha, a stable isosteric mimic of PGE2, was conjugated to two different protein immunogens and the immune response system was duped into producing antibodies with poor recognition for prostaglandins other than the hapten mimic (9-deoxy-9-methylene-PGF2alpha) and its isosteric counterparts (PGE1 and PGE2). With this procedure, crossreaction that would ordinarily arise from the chemical or metabolic instability of an authentic PGE2 immunogen was avoided. Antibodies raised against a keyhole limpet hemocyanin conjugate of 9-deoxy-9-methylene-PGF2alpha had an average intrinsic association constant, Ko = 2.6 X 10(9) liters.mole-1, for PGE2. Crossreaction was low for a number of related prostaglandins, and a sensitive radioimmunoassay procedure with a detection limit of 6 pg was developed.
